It overcomes the drawbacks of slow imaging detection or flow cytometry for multi-parameter detection, achieving fast and high-quality detection of large sample sizes. It also enables objective, multi-parameter data collection and analysis at the single-cell level, making it an ideal platform for cell function research and large-scale screening.
High-Content Imaging System Workflow
1. Cellular Morphological Analysis
Cellular morphological features are closely related to physiological and pathological processes. Using laser confocal high-content methods allows direct measurement of various morphological features of cells and their response to external environments or drugs. This approach aids in the direct discovery of potential therapeutic targets from a functional verification perspective. NBLST can provide cellular morphology and phenotype change analysis based on laser confocal high-content platforms, including nuclear morphology analysis, cell cytoskeleton rearrangement analysis, microtubule fiber/protein, stem cell clone analysis, lipid droplet detection, and three-dimensional cell imaging.
2. Signal Pathway Research
NBLST offers various signal pathway analysis modes. Using the high-content high-throughput imaging and screening platform, it is convenient and fast to analyze the activity changes of key factors in cellular signal pathways. It also allows large-scale analysis and screening of inhibitors of key factor activity. Examples include GPCR activation analysis, protein synthesis or expression, protein translocation, and transcription factor activation analysis.
3. Cell Toxicity Analysis
Cell toxicity analysis uses cells, tissues, or organs as experimental materials and is a new method to replace animal experiments for toxicity testing. Based on the high-content screening platform, NBLST has pre-built various cell state analysis modes for convenient, fast, and multi-index analysis of cell changes. It allows large-scale screening of small molecules, nucleic acid drugs, etc. NBLST can provide analysis projects such as live/dead cell detection, cell autophagy, cell autophagy, and DNA damage and repair.
4. Cell Proliferation, Apoptosis, and Cell Cycle Analysis
Cell proliferation, including cell growth and division, is a crucial feature of life activities. The complete process of cell growth and division is called the cell cycle. Detecting new DNA synthesis in cells or cell groups is a precise method to measure proliferation. NBLST can provide comprehensive cell proliferation and cycle analysis projects, such as cell cycle, cell proliferation, and mitosis.
Cell Cycle Analysis
1. Co-localization Analysis
In multi-fluorescent sample images, when two or more fluorescent probes are very close in the microscopic structure, emission signals often overlap, resulting in the co-localization effect. NBLST can provide services based on cell imaging, such as marker co-localization and biochemical detection, including Fluorescence In Situ Hybridization (FISH).
2. Tissue Analysis
With the development of modern medicine and pharmacology, the use of animal tissues and organs as drug screening models has become more common. NBLST's high-content platform is equipped with a unique tissue slice holder, which can be used to observe tissue slices of various organs and pathological types. It enables large-scale imaging screening of tissue and organ models, such as tumor 3D microtissue imaging analysis, angiogenesis, and tissue slice analysis projects
3D微组织分析
High-Content Screening Service Advantages
1. Efficient and Rapid: Experiment time shortened from months to days or even minutes.
2. High Throughput: Rapid large-scale cell biology analysis from single cells and subcellular structures to cell populations.
3. Accurate and Powerful: Achieved through multiple fluorescence labeling, systematic and rapid capture, high-resolution imaging, and robust software analysis.
4. Powerful Image Acquisition Capability: Wide-field scanning, laser confocal high-definition imaging, 3D imaging, live-cell dynamic imaging. Seamless stitching function is provided for perfect organ/tissue/cell images in different imaging modes.
5. Simple Operation: Fully automated throughout the process
Rich Technical Expertise
NBLST has accumulated years of practical experience in high-content imaging and analysis. We offer comprehensive high-content screening solutions, enabling efficient and rapid execution of various screening services.
Professional Technical Platform
NBLST boasts a professional technical service platform and currently offers high-content screening and analysis technology services. Our aim is to provide excellent single-cell analysis capabilities with the shortest data collection time, offering a more efficient and precise platform for cell function research and large-scale screening.
Service Range
