Simultaneously establishing a high-quality animal management system and a comprehensive breeding system, we aim to create a multifunctional experimental animal center integrating alpaca breeding, feeding, testing, analysis, technical research, and supply services. Our focus includes conducting comprehensive research on the scale breeding of alpacas, disease prevention, and control, as well as exploring the establishment of industry standards for alpaca breeding.

Antigen immunization is conducted in four rounds to achieve optimal immune effects, with an immunization cycle of approximately 50 days.

  1. Antigen preparation:

 Antigen preparation involves approximately 2-3mg, uniformly mixed and evenly distributed into 5 portions, with each portion being approximately 1mL. These portions are then frozen and stored at -80 degrees Celsius, allowing for a total of 4-5 immunization rounds.

  1. Alpaca immunization and blood collection:

    1. Immunization target: "Alpaca", preferably 3 years old, with similar effectiveness for both males and females.
    2. Immunization steps:
      1. Thaw the antigen from the -80°C freezer and place it on ice. After thawing, add 1mL of adjuvant, mix thoroughly, and emulsify.
      2. Inject near the lymph nodes on both sides of the neck. Administer 2 injections on each side, with approximately 1mL emulsified antigen per injection point. Each alpaca receives a total of approximately 2mL emulsified antigen per immunization.
    3. Blood collection procedure:
      1. First Large Blood Draw: Approximately one week after the third immunization, peripheral blood will be drawn to isolate serum. After testing the serum for antibody titers and confirming compliance with requirements, a 100mL sample of peripheral blood will be collected from the alpaca's jugular vein (PBMC collection).
      2. Second Large Blood Draw: Approximately one week after the third immunization, peripheral blood will be drawn to isolate serum. After testing the serum for antibody titers and confirming compliance with requirements, a 100-150mL sample of peripheral blood will be collected from the alpaca's jugular vein (PBMC collection).
      3. In case the antibody titers in the serum do not fully meet the requirements after the fourth immunization, an additional fifth immunization can be administered.

 

Operating Procedure:

Day 0:

First immunization injection, complete Freund's adjuvant mixed emulsification antigen.

Day 1:

First immunization injection, complete Freund's adjuvant mixed emulsification antigen.

Day 10:

Second immunization injection, incomplete Freund's adjuvant mixed emulsification antigen.

Day 19:

Third immunization injection, incomplete Freund's adjuvant mixed emulsification antigen.

Day 28:

Collect 100 mL of PBMC.

Day 28:

The fourth immunization injection, mix the incomplete Freund's adjuvant with emulsified antigen.

Day 43:

Collect 100-150 mL PBMC.