
Constructing a High-Diversity Nanobody DNA Library involves a two-phase blood collection process. The first round of blood collection occurs after the completion of the third antigen immunization, and the second round follows the fourth antigen immunization, with a total blood volume exceeding 200mL. Lymphocytes are isolated, and RNA...
Constructing a High-Diversity Nanobody DNA Library involves a two-phase blood collection process. The first round of blood collection occurs after the completion of the third antigen immunization, and the second round follows the fourth antigen immunization, with a total blood volume exceeding 200mL. Lymphocytes are isolated, and RNA extraction, along with the construction of the library, is performed.

Bacteriophage Surface Display Screening: Take an appropriate amount of constructed nanobody library, add bacteriophages after suitable cultivation. Nanobodies are displayed on the surface of bacteriophages in the form of fusion proteins with the pIII protein. After incubation with the antigen, washing, and elution, specific nanobodies are...
Bacteriophage Surface Display Screening: Take an appropriate amount of constructed nanobody library, add bacteriophages after suitable cultivation. Nanobodies are displayed on the surface of bacteriophages in the form of fusion proteins with the pIII protein. After incubation with the antigen, washing, and elution, specific nanobodies are preliminarily obtained