After obtaining the positive nanobody sequence, the sequence is constructed into an expression vector for expression and purification. Due to the relatively simple structure of nanobodies, they can be correctly expressed and folded in prokaryotic cells. The expression and purification are performed using Escherichia coli BL21.
Constructing a High-Diversity Nanobody DNA Library involves a two-phase blood collection process. The first round of blood collection occurs after the completion of the third antigen immunization, and the second round follows the fourth antigen immunization, with a total blood volume exceeding 200mL. Lymphocytes are isolated, and RNA extraction,…
Constructing a High-Diversity Nanobody DNA Library involves a two-phase blood collection process. The first round of blood collection occurs after the completion of the third antigen immunization, and the second round follows the fourth antigen immunization, with a total blood volume exceeding 200mL. Lymphocytes are isolated, and RNA extraction, along with the construction of the library, is performed.
Our company currently operates a large-scale alpaca breeding base in the Donghu High-tech Zone of Wuhan. With a capacity for immunizing up to 100 adult animals annually, it is well-equipped to meet the demand for a substantial volume of antigen immunizations.
纳米抗体可以基于BRET方法，实时检测受体活性状态。根据相关文章记载，构建相对应的功能质粒，并做检测，得到的结果和文章中一致，基于BRET技术，纳米抗体可以实时检测受体的活性状态。